α-GLUCOSAMINIDASE

Studies on glucosaminidase. 2. Substrates for N-acetyl-beta-glucosaminidase.

the oxygen uptake and acetoacetate oxidation when added alone or with fumarate, but not in the presence of a-oxoglutarate. The reasons for these differences are discussed. Anaerobically, relatively slight inhibitions of the reduction of acetoacetate were observed. 6. The oxidation of L( + )-p-hydroxybutyrate is inhibited by dinitrophenol, whereas that of the D(-)-form is not. This is related to...

Studies on glucosaminidase; N-acetyl-beta-glucosaminidase in rat kidney.

SpectrophotometricAssayfor UrinaryN-Acetyl-f3-D-Glucosaminidase Activity

An improved assay for N-acetyl-/3-o-glucosaminidase activity in urine is described that involves (a) gel filtration to separate the enzyme from inhibitors in urine, (b) enzymic hydrolysis of p-nitrophenyl-N-acetyl-3-D-glucosaminide at pH 4.4, and (C) spectrophotometry of the liberated p-nitrophenylate. Measurements of activity of the enzyme in 58 urine specimens correlated closely (r = 0.9998) ...

Urinary biomarker N-acetyl-β-D-glucosaminidase can predict severity of renal damage in diabetic nephropathy

BACKGROUND Diabetic nephropathy is a clinical diagnosis where proteinuria is present in a patient with diabetes. Early intervention can significantly improve the prognosis. However, imprecision of the currently available biomarkers have impaired effective therapies in a timely manner. Urinary N-acetyl-β-D-glucosaminidase (NAG) is excreted in abnormally high amounts in many renal diseases. The a...

Occupational exposure to benzene, toluene and xylene and the lymphocyte lysosomal N-acetyl-beta-D-glucosaminidase.

In 108 workers occupationally exposed to benzene, toluene and xylene the numbers of lymphocytes containing intact lysosomes having cytochemically determined activity of N-acetyl-beta-D-glucosaminidase (NAG) were calculated in the peripheral blood. The benzene, toluene and xylene concentration in the air of at work places were 0-370, 0-580 and 0-506 mg/cu. m, respectively. The phenol concentrati...